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Original Research Article | OPEN ACCESS

Inhibition of Lipopolysaccharide-Induced iNOS, COX-2, and TNF-α expression by Aqueous Extract of Orixa Japonica in RAW 264.7 Cells via Suppression of NF-KB Activity

Chang-Hee Kang1, Yung H Choi2, Il-Whan Choi3, Jae-Dong Lee4, Gi-Young Kim1

1Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756; 2Department of Biochemistry, College of Oriental Medicine, Dongeui University, Busan 614-054; 3Department of Microbiology, Inje University College of Medicine, Busan 614-735; 4Department of Microbiology, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea.

For correspondence:-  Gi-Young Kim   Email: immunkim@jejunu.ac.kr   Tel:+82647543427

Received: 16 September 2010        Accepted: 19 January 2011        Published: 20 April 2011

Citation: Kang C, Choi YH, Choi I, Lee J, Kim G. Inhibition of Lipopolysaccharide-Induced iNOS, COX-2, and TNF-α expression by Aqueous Extract of Orixa Japonica in RAW 264.7 Cells via Suppression of NF-KB Activity. Trop J Pharm Res 2011; 10(2):161-168 doi: 10.4314/tjpr.v10i2.7

© 2011 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the anti-inflammatory effects of aqueous extract of Orixa japonica (AEOJ) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
Methods: The expression of mRNA and protein using RT-PCR and Western blot analysis was investigated. The level of nitric oxide (NO) production was analyzed using Griess reaction. Release of prostaglandin E2 (PGE2) and tumor necrosis factor-α (TNF-α) was determined using sandwich ELISA.
Results: AEOJ potently inhibited the production of nitric oxide (NO), prostaglandin E2 (PGE2), and tumor necrosis factor-α (TNF-α) in LPS-stimulated RAW 264.7 cells. Consistent with these findings, AEOJ was also found to significantly reduce LPS-induced expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and TNF-α at the transcriptional level. Additionally, AEOJ attenuated LPS-induced NF-κB activity via the inhibition of IκB phosphorylation and degradation. It was also found that the NF-κB inhibitor N-acetyl cysteine (NAC) attenuated LPS-induced gene expression of iNOS, COX-2, and TNF-α. These results indicate that AEOJ attenuates LPS-induced inflammatory mediators such as NO, PGE2, and TNF-α via suppression of NF-κB activity.
Conclusion: These results suggest that AEOJ has a potential activity to alleviate LPS-induced inflammation.

Keywords: Orixa japonica, Nitric oxide, Prostaglandin E2, Tumor necrosis factor-^5;, Nuclear factor-_4;B

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